Journal: Molecular Biology Reports
Article Title: Macrophages regulate healing-associated fibroblasts in diabetic wound
doi: 10.1007/s11033-023-09100-1
Figure Lengend Snippet: M1/proinflammatory macrophages regulate the phenotype of fibroblasts. A Fibroblasts isolated from non-diabetic wound were cultured alone, or with isolated M1 macrophages from non-diabetic wound, or with isolated M1 macrophages from diabetic wound. ELISA for MMP1, MMP3, MMP11, CHI3L1 and VEGF-A in fibroblasts was performed. B Fibroblasts isolated from diabetic wound were cultured alone, or with isolated M1 macrophages from diabetic wound supplied with/without IL6, or with isolated M1 macrophages from non-diabetic wound. ELISA for MMP1, MMP3, MMP11, CHI3L1 and VEGF-A in fibroblasts was performed. *p < 0.05. NS non-significant. NDW non-diabetic wound. DW Diabetic wound
Article Snippet: Enzyme-linked immunosorbent assays (ELISAs) were done for IL1β (ab197742; Abcam), tumor necrosis factor alpha (TNFα, ab208348; Abcam), interferon gamma (IFNɣ, ab282874; Abcam), IL6 (Ab100713; Abcam), IL10 (M1000B; R&D Biosystems), CD163 (ab272204; Abcam), transforming growth factor β1 (TGFβ1, ab119557; Abcam), VEGF-A (ab209882; Abcam), fibroblast growth factor 1 (FGF1, ab223587; Abcam), MMP1 (NBP3-06885; Novus Biologicals, Centennial, CO, USA), MMP3 (ab203364; Abcam), MMP11 (NBP3-06935; Novus Biologicals) and CHI3L1 (ab238262; Abcam).
Techniques: Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay